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DNA Transfection: Calcium Phosphate Method

Source: link.springer.com

Calcium phosphate mixes with DNA to form tiny particles that cells easily swallow.
This cheap, straightforward technique works well for adding genes to brain cells grown in dishes.
It avoids harsh chemicals, making it safer for delicate nerve cells despite some toxicity risks.

Calcium phosphate transfection delivers DNA plasmids into cells by creating a precipitate that cells take up naturally. It stands out for its low cost and ease, especially in primary neuron cultures where other methods can harm sensitive cells. This protocol optimizes the process to boost gene expression while minimizing neuron damage.